FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7428910
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC87310.11752733577335032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA34050.023.8542464
GAGGTCA36650.018.403652
TAATCCC46400.017.4025069
AGGTCAT41600.017.0129493
TCTAGAC60650.016.9945537
CTAGACT60700.016.7458028
GTCATAA51700.015.6187075
ATTCTAG75250.015.2123825
TTCTAGA74400.014.5562196
TAGACTT71400.014.5023749
CGGGAAT38150.014.2015831
CATTCTA84650.013.7475664
GGGGAAT18500.013.3585121
CGAGGTC51550.013.1835941
TGGGGGG22450.013.1250751
CCGGGAT23250.012.8778711
ATAATCC67900.012.3818168
AGTGACC21150.011.9028846
GGGAATC31700.011.5377482
TCATTCT110850.010.4125543
Produced by FastQC (version 0.11.5)