FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934099
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC185880.20805679453518483No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA147190.1647508047537866No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT142410.15940051705269886No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT124730.13961116840097698No Hit
TTTTAATTTAATTAATATTATTATTTTCTAAAAATATAATAATCATTCAT109000.12200446849760677No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT107560.1203926663449778No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA106000.11864654734629648No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT105840.11846745821822659No Hit
CTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTAGAAA104650.11713548282820685No Hit
TTTTAAATGAAGTCTAGAATGAAGGAGCTAATGAGATTTTAACTTTATAA104180.11660940851450158No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA100920.11296046753007773No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA99140.1109681009803003No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT98050.10974805629532425No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC96470.10797955115563417No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA95320.10669234804763189No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT91290.10218154063437175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA61500.027.5728154
GAGGTCA68250.023.1759152
AGGTCAT70850.022.1239223
GTCATAA81750.021.091475
TAATCCC82200.020.975779
CGAGGTC80650.019.32981
TCTAGAC114650.016.6546367
CTAGACT114450.016.6007378
TGGGGGG26650.016.407731
ATAATCC107600.016.2449638
TAGACTT124950.015.2817459
TTCTAGA134250.014.6124746
ATTCTAG137700.014.487835
TCATAAT129600.013.5607826
CATTCTA149250.013.5257884
AGTGACC25150.013.2205066
TGACCGC26700.012.6307818
CGGGTCT19800.012.4823421
AATCCCA89350.012.22690210-11
GGGGAAT20950.012.0240211
Produced by FastQC (version 0.11.5)