FastQCFastQC Report
Tue 11 Dec 2018
H5FFKAFXY_n01_Ant-Tm1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5FFKAFXY_n01_Ant-Tm1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12313849
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC206770.16791662785535214No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA162750.13216826030593684No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT158350.12859504773852595No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT149310.1212537201000272No Hit
TTTTAAATGAAGTCTAGAATGAAGGAGCTAATGAGATTTTAACTTTATAA126660.10285979631551435No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT125160.10164165566753337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA74050.024.1187464
GAGGTCA77000.021.0359752
TAATCCC91500.019.6225579
AGGTCAT85650.018.689293
TCTAGAC125250.017.8239947
CTAGACT124850.017.652838
GTCATAA105400.017.4406365
CGAGGTC95700.016.6351181
TGGGGGG36600.016.6196521
TAGACTT145450.015.446589
TTCTAGA153750.014.8916
ATTCTAG157550.014.8333295
ATAATCC130550.014.1169148
CATTCTA170300.013.8064654
CGGGAAT54650.012.7826291
GGGGAAT24800.011.8804541
GGGAATC39900.011.3096652
TCATAAT170500.010.9764986
TCATTCT226800.010.8905673
CGGGCAT27250.010.8123041
Produced by FastQC (version 0.11.5)