FastQCFastQC Report
Tue 4 Feb 2020
H5CM7BGXF_n02_Salt_NSF_Plate_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CM7BGXF_n02_Salt_NSF_Plate_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397715679
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42115071.0589240561471553No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT11460790.2881654057193958No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC6603410.16603343415083216No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5786020.14548131505773498No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4356090.1095277412988287No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT4126360.10375150435042316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA933850.021.7805391
GTATCAA3185500.020.4368971
GGACCAT2951650.019.6999766
GTTATAC3626850.018.8974443
GGGGGTA3603850.018.2856921
ATACCGG3801350.018.1805296
TGGACCA3407750.018.1632695
TCAGGGG3633300.018.0569318
GGGGTAT3603950.018.03175522
GTAGATC1617400.018.02728743
TAGATCT1587350.017.9870444
GTACTGG2580250.017.9835261
GGGTATT3695850.017.62149623
TACCGGT3900800.017.6207547
GGTATTC3669550.017.61884524
TGTTATA3984150.017.5226082
TGGCATA3575150.017.38359536
CAGGGGG3775600.017.37804219
TATACCG4039750.017.3756035
AGGGGGT3783350.017.36922320
Produced by FastQC (version 0.11.5)