FastQCFastQC Report
Tue 4 Feb 2020
H5CM7BGXF_n01_Salt_NSF_Plate_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CM7BGXF_n01_Salt_NSF_Plate_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397715679
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG59909051.506328595106757No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA6026650.013.87700752
CTTCGTC8508200.013.8683732
AACTACC5044050.013.8581026
TCAGAAC5051950.013.8488422
TTCCACC3740300.013.8446752
CCTCTCT3290350.013.8341774
CCACACT3717500.013.8275292
CCACTCT4640900.013.825875
ACACCTT3774300.013.8254882
TCAACCG3514550.013.8247981
TCCACAC3701500.013.8182621
TCGACTT3900200.013.8128196
GTCAGCT8479000.013.8111786
TTCGACC6025850.013.8072971
AGACGTC5455500.013.8039581
GGTCCAA5188500.013.8029743
CTCCTAC2996900.013.8018754
TCCTTAC3535450.013.7985144
AATCCTT3531250.013.7932562
CCTCAGT3573800.013.7906646
Produced by FastQC (version 0.11.5)