FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39535956
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG771830.19522229334735197TruSeq Adapter, Index 14 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG137700.052.35952443
CTCGTAT135250.051.9309242
TCTCGTA137500.051.0311641
CGTATGC145550.050.438844
TATGCCG146900.049.83833346
ATGCCGT147550.048.64032747
ATCTCGT153450.046.06429740
TGCCGTC165750.043.4244948
CCGTCTT168300.042.8482850
GCCGTCT174800.040.84068749
CATCTCG172000.040.61469739
CGTCTTC183450.039.28990651
GTATGCC201100.036.91945645
GCTTGAA252950.028.93561459
TGCTTGA285500.025.45582858
GTTCCAT310800.024.19642335
ACAGTTC366750.020.86203432
TTCCATC377800.019.42145536
GTCTTCT389850.018.92238652
CTGCTTG405550.018.29483457
Produced by FastQC (version 0.11.2)