FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36975203
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT70850.041.14283842
TCGTATG74000.039.85825343
TATGCCG77750.038.11779446
CGTATGC78000.038.0370244
TCTCGTA77500.037.61256841
ATGCCGT79650.037.21173547
AATCTCG84150.033.9436839
ATCTCGT89200.032.67934840
TGCCGTC96750.030.52782848
CCGTCTT100700.029.22709750
GCCGTCT109500.026.91016249
CGTCTTC117700.025.18098851
GTATGCC126150.023.76604745
GCTTGAA180000.016.79406759
TGCTTGA207750.014.60074658
GTCAAAT184500.014.45302535
CAAATCT227650.012.44078137
AAATCTC228600.012.26852338
TCAAATC229250.012.08313136
CAGTCAA231650.011.86843533
Produced by FastQC (version 0.11.2)