FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30944462
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG383280.12386061195699574TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT79050.042.9847542
TCGTATG79850.042.85664743
TATGCCG82200.041.59510446
TCTCGTA83750.040.81974841
CGTATGC84200.040.5219444
ATGCCGT86900.039.3090347
ATCTCGT95850.035.5950740
CCGTCTT99050.034.8007550
TGCCGTC99050.034.6964148
GCCGTCT105150.032.65077649
CGTCTTC110750.031.40387251
CATCTCG111200.030.6813939
ACGGCTA122950.027.91620332
GTATGCC126000.027.46373245
CGGCTAC129450.026.19498633
GCTTGAA166600.021.25162159
TCACGGC165000.020.90616430
CACGGCT172400.020.14882931
GTCACGG173350.020.03902429
CTACATC187350.018.59708636
Produced by FastQC (version 0.11.2)