FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29697710
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49450.044.99662843
CTCGTAT51400.044.36288542
CGTATGC55950.042.36046244
TATGCCG59050.041.48364346
TCTCGTA56600.040.8356841
ATGCCGT60900.039.3765347
TATCTCG62350.039.33827239
ATCTCGT67700.035.21056740
TGCCGTC79150.030.90759748
CCGTCTT81650.030.3412250
GCCGTCT88000.027.52476549
GTATGCC94450.026.66487745
CGTCTTC97000.025.96660651
GCTTATC116450.025.80213736
TATCGCG13700.025.1803439
TCGCGTA10100.021.8594941
TAGCTTA147100.021.01231234
CGCGTAT10450.020.7972442
AGCTTAT150650.020.35694335
ACTAGCT166400.018.57481232
Produced by FastQC (version 0.11.2)