FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28291183
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG26515719.372428858842701TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3478350.064.7888242
TCTCGTA3473150.064.774841
TCGTATG3513700.064.7722243
GATCTCG3460700.064.7708239
CGTATGC3514300.064.72687544
ATGCCGT3510450.064.7254547
GCCGTCT3506250.064.7007549
TGCCGTC3510050.064.6916548
TATGCCG3514050.064.6763346
CCGTCTT3509750.064.6556750
CGATCAG3477450.064.6477833
CGTCTTC3511450.064.5916751
ACGATCA3497350.064.56824532
ATCTCGT3477650.064.55665640
CACGATC3504100.064.4782131
GTCACGA3524100.064.3781729
TCACGAT3516300.064.3658130
GTATGCC3547350.064.0986945
AGTCACG3554500.064.0695628
CGTCTGA3615350.063.84595516
Produced by FastQC (version 0.11.2)