FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25541628
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7430182.9090471445281407TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG277710.10872838645993904TruSeq Adapter, Index 8 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG1171400.065.7196539
CGCGTAT110950.064.9841342
TCGCGTA112400.064.9743641
CGTCTGA1588600.064.4419216
ACGTCTG1594950.064.2143715
TCTCGTA1136500.064.05028541
ACACGTC1605850.064.0446113
CTCGTAT1132900.063.96161742
CGTATGC1181800.063.96055644
TCGTATG1104100.063.8019543
TATGCCG1203700.063.78581646
ATGCCGT1140750.063.74535847
CACGTCT1611550.063.7001114
AATCGCG176950.063.51621239
TGCCGTC1153300.063.50945348
CCGTCTT1173700.063.43933550
ATCTCGT1177700.063.394340
GCCGTCT1131500.063.39380349
CGTCTTC1169000.063.25826651
ATCGCGT131850.063.23885740
Produced by FastQC (version 0.11.2)