FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32378766
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG16266295.023752294945397TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1383000.42713178136560237TruSeq Adapter, Index 3 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG413630.12774730204356768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2189650.065.8591342
TCTCGTA2195750.065.8068741
TCGTATG2169100.065.7911543
CGTATGC2220850.065.7428144
ATGCCGT2173550.065.6888947
GCCGTCT2155550.065.5700449
TATGCCG2226350.065.5054946
TGCCGTC2177450.065.50310548
CCGTCTT2190650.065.47864550
CATCTCG2240650.065.4424439
ATCTCGT2230200.065.4399140
CGTCTTC2174950.065.3709451
GTATGCC2275350.064.5719845
CGTCTGA2525150.064.22542616
ACGTCTG2538550.063.92531615
ACACGTC2555050.063.7257113
CACGTCT2561750.063.45776414
GCTTGAA2266850.063.45733359
CTTAGGC2487200.063.39535533
TAGGCAT2491450.063.0110835
Produced by FastQC (version 0.11.2)