FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34462320
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG642426718.641423444504028TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG7259172.1064078100371653TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG480960.139561120667442TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG446220.12948054570905268TruSeq Adapter, Index 1 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380440.11039303215802071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG350640.10174590683389859TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8417700.066.6367651
ATCGGAA8550000.066.597422
TCGGAAG8531500.066.5523453
CGGAAGA8549100.066.3230744
ACACGTC8483200.066.1008613
CACGTCT8481850.066.0064314
CGTCTGA8468850.065.98762516
CGATCTC8175900.065.9856238
ACGATCT8157300.065.9746937
ACGTCTG8473500.065.9688715
GCACACG8506050.065.9638311
GATCTCG8204850.065.9550239
CACGATC8143200.065.9542736
CACACGT8506000.065.9314812
TCACGAT8220700.065.8388335
TCTCGTA8269800.065.7487641
CTCGTAT8294550.065.737742
ATCACGA8257650.065.7333134
ATGCCGT8361100.065.7139847
TGCCGTC8354350.065.7042648
Produced by FastQC (version 0.11.2)