FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33595981
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG17589295.235533976519394TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1929620.5743603676880279TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2334200.066.3831442
TCTCGTA2336850.066.3743941
AATCTCG2342250.066.2646739
TCGTATG2369650.066.24711643
ATGCCGT2344450.066.23492447
CGTATGC2381000.066.2167344
GCCGTCT2330850.066.1421649
TGCCGTC2344300.066.1277948
TATGCCG2380450.066.0995746
CCGTCTT2344850.066.0414550
ATCTCGT2355800.066.02859540
CGTCTTC2338000.065.8709151
GTATGCC2433300.064.948445
GTAATCT2411500.064.4637
CGTCTGA2567000.064.0588716
GCTTGAA2419300.063.91967459
ACGTCTG2580050.063.7621715
TGTAATC2456950.063.6162636
ACACGTC2596450.063.53032713
TAATCTC2442750.063.52153838
Produced by FastQC (version 0.11.2)