FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33520131
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG33289849.93129770286399TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT4290400.067.11339633
CTCGTAT4318750.067.0568342
TATCTCG4313700.067.0566739
CGTATGC4328350.067.0558644
TCGTATG4328000.067.053543
ATGCCGT4313400.067.0325447
TCTCGTA4324050.067.0299941
TATGCCG4325750.067.0148446
GCCGTCT4296900.067.0117149
CCGTCTT4301800.066.99763550
TGCCGTC4309350.066.99515548
CGTCTTC4292450.066.96342551
ACGCCAA4314150.066.95431532
GTCACGC4337000.066.9166329
ATCTCGT4339600.066.8584740
TCACGCC4330150.066.8077930
CACGCCA4332850.066.72108531
AGTCACG4373100.066.7112328
GTATGCC4366700.066.4841545
CGTCTGA4438350.066.3932116
Produced by FastQC (version 0.11.2)