FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38020399
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG34636479.109970150497368TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2789610.7337140254630152TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2714580.7139798822205943TruSeq Adapter, Index 5 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4425600.067.6490441
CTCGTAT4418150.067.6483442
GATCTCG4451450.067.5633539
TCGTATG4978900.067.4732743
ATCTCGT4443500.067.461540
CGTATGC5135750.067.4198344
ATGCCGT5019250.067.4005147
CCGTCTT5019200.067.3778950
TGCCGTC5007600.067.369948
GCCGTCT4963700.067.36722649
TATGCCG5130650.067.3664846
CGTCTTC4986050.067.2711251
GTATGCC5202350.066.841545
CGTCTGA5569900.066.6352216
ACGTCTG5582950.066.505615
ACACGTC5598700.066.4462313
CACGTCT5603400.066.3288514
AGTGATC4661100.066.3223336
GCTTGAA5107750.066.23810659
GTGATCT4560450.066.13142437
Produced by FastQC (version 0.11.2)