FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26512178
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG19858617.490372914665857TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2398250.9045843008446911TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG2575350.066.3137239
CTCGTAT2597600.066.3115342
TCTCGTA2593750.066.3011541
TCGTATG2634650.066.2752543
ATGCCGT2626250.066.23707647
CGTATGC2635300.066.2268144
TGCCGTC2625350.066.1518848
GCCGTCT2619300.066.1293549
TATGCCG2636050.066.10943646
CCGTCTT2622750.066.0907850
ATCTCGT2600950.066.0454140
CGTCTTC2619400.065.9890351
CGTCTGA2711350.065.4216416
GTATGCC2667950.065.4099945
ACGTCTG2726100.065.10374515
ACCAATC2600500.065.0293136
ACACGTC2737650.064.9523113
GACCAAT2638750.064.9308335
CACGTCT2741000.064.81264514
CCAATCT2629000.064.59334637
Produced by FastQC (version 0.11.2)