FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35005602
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG8349462.385178235186471TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG578430.16523926656082075TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1174350.066.53464541
CTCGTAT1173300.066.4408342
TCGTATG1162500.066.4229643
CGTATGC1211250.066.33916544
ATGCCGT1174250.066.0763647
TATGCCG1228900.066.0373246
ATCTCGT1219250.065.8755140
TGCCGTC1188200.065.86433448
GCCGTCT1169400.065.82567649
CATCTCG1245900.065.6100439
CCGTCTT1186500.065.59247650
CGTCTTC1190500.065.292851
GTATGCC1276600.063.9488145
CGTCTGA1514200.063.12379516
GATCATC1479200.062.68622236
TCGCGTA79000.062.5743441
ACGTCTG1531800.062.45392215
ACACGTC1543750.062.0555613
CACGTCT1549750.061.78343614
CGCGTAT79900.061.39414242
Produced by FastQC (version 0.11.2)