FastQCFastQC Report
Mon 11 Dec 2017
H5CL7BGX5_n01_mb61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CL7BGX5_n01_mb61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34770572
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14923244.2919167392472TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1308320.37627221087993606TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2000400.066.7510443
ATGCCGT2050600.066.72455647
CGTATGC2090950.066.7213144
CTCGTAT2023350.066.677542
TATGCCG2105500.066.6603946
CCGTCTT2060150.066.6211650
TCTCGTA2032750.066.5917741
GCCGTCT2033450.066.5496549
TGCCGTC2058150.066.485348
CGTCTTC2050250.066.4063451
TATCTCG2086900.066.2691539
ATCTCGT2072200.066.0590340
CGATGTA2311900.065.7494334
CCGATGT2316850.065.6105133
ACCGATG2324900.065.5877132
CACCGAT2332500.065.5262431
GTATGCC2166800.065.326345
TCACCGA2354700.065.068630
GTCACCG2358250.065.0280129
CGTCTGA2402700.064.7630616
Produced by FastQC (version 0.11.2)