FastQCFastQC Report
Fri 31 Jan 2020
H5CKCBGXF_n02_Salt_NSF_Plate_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CKCBGXF_n02_Salt_NSF_Plate_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354015844
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45466421.28430466518894No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT9048960.25560889867968734No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5150560.14548953351364693No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG4523240.12776942265894745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA776350.021.1287821
TAGATCT1724300.020.1797844
GTATCAA2683900.020.1539481
GTAGATC1814000.019.57360543
GGACCAT2580500.019.4117076
GTTATAC2823500.018.627153
GGGGGTA2826100.017.93139521
TGGACCA3000300.017.9153585
TCAGGGG2848650.017.70615618
GTACTGG2209700.017.6827961
ATACCGG3049150.017.636696
GGGGTAT2838000.017.62514522
GGGTATT2848850.017.57946423
TATACCG3098900.017.4866035
TACCGGT3036900.017.4182327
GGTATTC2884600.017.25851224
TGTTATA3117100.017.2545412
CAGGGGG2929800.017.19846719
TGGCATA2798750.017.10081536
AGGGGGT2982750.016.94105720
Produced by FastQC (version 0.11.5)