FastQCFastQC Report
Fri 31 Jan 2020
H5CKCBGXF_n01_Salt_NSF_Plate_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CKCBGXF_n01_Salt_NSF_Plate_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354015844
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG61235961.729751959915105No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCTT4076950.013.86624052
CGCATCC4551400.013.8606095
TTCCACC3943700.013.8595912
CCTCTCT2564950.013.8426334
TCGACCA4257200.013.8424722
CTTCGTC5744450.013.8420672
AGACATC4595800.013.8406732
CCTCAGT4141100.013.8389346
AGAGACT6762050.013.8324075
CGTTAGA7506150.013.8323031
TCAACCG3340300.013.8302381
AGACCTT7429450.013.8251145
CTCCTAC2944800.013.8208014
TCGCATC4566800.013.8093384
TAGACCT7490850.013.8078044
TACACCT4099800.013.8052721
ACATCGC4588450.013.8036754
CCTACGT2939350.013.7943366
CCACACT2898800.013.7935282
AACCGAT3357700.013.7933533
Produced by FastQC (version 0.11.5)