FastQCFastQC Report
Thu 24 May 2018
H5CHJBGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CHJBGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382434139
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19791350.5175100228172883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGTCGGGA7044680.18420635820904052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGG6619560.17309019579970084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT18924250.075.131911
AGCAGTG21137350.067.243032
CAGTGGT26435900.053.734824
GCAGTGG26566050.053.5673643
AGTGGTA27044350.052.542215
GTGGTAT31684300.044.8445056
TGGTATC31755750.044.67337
GGTATCA32237700.044.0387468
GTATCAA32822800.043.2375349
CAACGCA32944400.024.08849512-13
ATCAACG33369550.023.82853910-11
AGAGTAC33313950.023.72833418-19
GCAGAGT33489850.023.67631316-17
AGTACAT33422900.023.55041120-21
ACGCAGA33906850.023.46440714-15
TACATGG33641650.023.29816422-23
TATCAAC32924750.021.6334310-11
CAGAGTA33110150.021.43169818-19
CGCAGAG33317350.021.39719216-17
TCAACGC33326450.021.33972712-13
Produced by FastQC (version 0.11.2)