FastQCFastQC Report
Thu 24 May 2018
H5CHJBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CHJBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382434139
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTTCATCTTTCCCTCGCGGTACT18050560.4719913354806433No Hit
TCCAGCACCGGGCAGGTGTCAGACTC9003940.2354376631632251No Hit
TTTCATCTTTCCCTCGCGGTACTTGT6534810.17087412795017237No Hit
TTTTCATCTTTCCCTCGCGGTACTTG5829300.1524262456077437No Hit
CTGGTCTGCATCGTTTATGGTTGAGA5241420.13705418699558095No Hit
CCTTCCCCTAAATGATAAGGTTCAAT5050840.13207084527566196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACGA1778200.017.9444078
AGGACGA777200.017.8885978
GCGCGAT1042650.017.828361
TGACTAG1423950.017.82611
GGCTCGA1397150.017.751371
GACTGCG1513600.017.7331161
GGCGACT1357800.017.5696621
ATAGACC1155700.017.5315111
GCGTATG1550700.017.4776252
GATCTAG1723700.017.444841
GTCGAAC1225450.017.4176798
TACGCCG1321250.017.41031610
CGAGCGA609100.017.395146
CGGACTG1180050.017.3053761
ATGGCCA1198900.017.2984163
ATCGAGT1249400.017.2959121
CGGAGCT1264100.017.1913171
GGGAATG926900.017.171281
ACGAGCC1119700.017.0973321
CGTATGT1587250.017.0586853
Produced by FastQC (version 0.11.2)