FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_flywt0718t.351000000f38e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_flywt0718t.351000000f38e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15944853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC355410.2228995149720101TruSeq Adapter, Index 3 (100% over 50bp)
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG174740.10959022325260698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA104450.018.4173168
GTACTAA108500.017.3796339
TATGCCG127750.015.59735246-47
TTCGTAC126950.015.2306756
CGTATGC133500.015.06787444-45
CCTTTCG126850.014.9812273
CTTGAAA143750.014.07607560-61
CTTTCGT137050.014.0392634
CGCGGGG23000.013.8415081
CTCGTAT147850.013.68573342-43
CGGGGAT50100.013.6571851
GTATGCC137500.013.610476544-45
GCCGTCT136550.013.56602948-49
GGGGTAT31700.013.3403491
TCGTATG139300.013.33231242-43
TCCTTTC148000.013.1615032
TTTCGTA151450.013.0804975
CCGTCTT162000.012.59296450-51
TGCTTGA163500.012.56458658-59
GTCCTTT156950.012.5638651
Produced by FastQC (version 0.11.2)