FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_flywt0629t.351000000f38a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_flywt0629t.351000000f38a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18801876
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA111100.018.3838068
GTACTAA111450.018.2834559
TTCGTAC130700.016.13866
CCTTTCG138350.015.2125713
CTTTCGT147150.014.1410064
TCCTTTC160000.013.6591232
CGCGGGG30850.013.5540131
ACCTAGC15850.013.18808656
GTCCTTT163200.013.1600851
TTTCGTA166850.013.0690775
GACCTAG16100.012.9833035
CGGGGAT66350.011.2434381
GGGGTAT33000.011.0870811
ACTCTAG32100.010.6557955
GGGGTCT39250.010.5322231
CGGGTCT61200.010.4814831
TCGTACT221300.010.2595317
CGCAAAT151650.010.0891341
TACACCC151700.010.05258755
GGGGGAT55950.09.9363351
Produced by FastQC (version 0.11.2)