FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_flyorco0718t.351000000f390e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_flyorco0718t.351000000f390e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083645
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG164180.11657493496889478No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT141170.1002368349954859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA98400.018.1981858
GTACTAA100250.017.7675979
GGGGTCT27200.016.7702051
TTCGTAC113400.016.0868456
CCTTTCG118900.015.1437963
CGGGGAT48950.014.8516511
CTTTCGT122150.014.6237734
GGGTCTT106500.013.8738632
TTTCGTA134500.013.7397885
TCCTTTC135050.013.4738232
GTCCTTT137450.012.8943691
CGCGGGG23100.012.3417081
CGGGTCT43050.012.2514021
CGGGGGT22850.012.0608461
GCCGGTT45450.012.0226441
TACACCC137900.011.6440795
CTCGTCT121250.011.5955619
GCTACAC139250.011.4636123
GCGGGTT32750.011.4617731
CGGCTAC150100.011.3961411
Produced by FastQC (version 0.11.2)