FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antwt0629t.351000000f38bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antwt0629t.351000000f38bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19825385
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA311470.15710665896273893No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC244810.12348310007598844TruSeq Adapter, Index 2 (100% over 50bp)
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT239410.12075931942809685No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT239030.12056764597509707No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT223860.11291583996981647No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT208310.1050723605115361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG58050.027.6617265
GGTCATA106500.023.9105054
AGTGACC58300.022.1648676
CTCTAGT74900.021.5656196
GAGGTCA104800.021.0805362
CTAGTTG76950.020.8635658
TGACCGC67350.019.8175138
CACCTTA95000.019.703235
TAATCCC130100.019.4594429
AGGTCAT117600.018.9876273
TCTAGTT96750.018.2630147
CAGTGAC75300.017.5393945
CCTTAGA106050.017.1541257
CGAGGTC133750.016.4840741
GTACTCT99350.016.402363
CTTAGAG112550.016.247848
GTCATAA160650.015.9394315
TGTACTC108400.015.2962912
GTGACCG88750.015.0925017
TCCACCT126050.015.0386963
Produced by FastQC (version 0.11.2)