FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort8.351000000f3a0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort8.351000000f3a0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113395
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA55400.019.2947794
TAATCCC73000.014.3797149
GTCATAA79350.013.4110245
AGGTCAT66250.012.7647393
GGGGAAT31850.011.1888791
CGGGGGG26350.010.8194541
GAGGTCA80100.010.7951572
CGGGAAT65050.010.5914511
CTCGGGT60600.010.271491
CGGGTAT43600.010.0261951
ATAATCC106850.09.9131398
GGGAATC48650.09.5704722
CGGGGCT20450.09.5263221
GACCCTA31850.09.3953297
GGGGGAT32900.09.3875491
CCCGTAT26900.09.1851411
CCCAATT78400.09.0303571
TCGGGTA61200.08.9276372
GGGGTCA29900.08.8982982
TCTAGAC71700.08.8770067
Produced by FastQC (version 0.11.2)