FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort7.351000000f39d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort7.351000000f39d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15269513
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA58600.016.6198394
TAATCCC75350.013.4269499
CGGGGCT26550.011.4535881
AGGTCAT68600.011.3579963
GTCATAA87450.011.2453395
GGGGAAT44150.011.084921
GGGGCTA9256.002665E-1110.272632
CCCGGAT43900.09.9574781
CGGGTAT45550.09.5967791
TCTAGAC69700.09.4724657
CTAGACT70150.09.005448
CGGGAAT70050.08.8856511
ATAATCC114550.08.8321318
CGTACAA150450.08.8099625
GGGAAAT95350.08.720541
CCCTAAG63750.08.6801454-55
CGGGGAT72050.08.6389971
CGGGGGG27250.08.543891
TTCGTAC139700.08.5020873
GAGGTCA95050.08.397512
Produced by FastQC (version 0.11.2)