FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort6.351000000f39c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort6.351000000f39c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29534172
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA111850.017.4997184
TAATCCC143250.013.5283989
AGGTCAT126850.012.6217483
GTCATAA165000.011.8048415
GAGGTCA171300.010.8999762
CGGGAAT128900.010.579621
CCGGGAG179200.010.2085461
CTAGACT144500.010.0585278
TCTAGAC148450.09.9508697
ATAATCC207500.09.7286298
CGGGGCT48200.09.6609561
GGGGAAT70300.09.5978541
GACCCTA69000.09.43088157
CCGGAAT105650.09.1299351
GGGAATC100450.09.0811662
ATTCTAG191350.09.0620125
CGGGGGG54350.09.0048981
CCGGGAT151450.09.0043791
GTTTAAT288650.08.9879821
CGGCTCT139300.08.9711021
Produced by FastQC (version 0.11.2)