FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort5.351000000f39af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort5.351000000f39af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18815750
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA86950.020.4348874
TAATCCC108650.015.9129929
AGGTCAT95750.015.1832393
GTCATAA122000.014.4468365
GAGGTCA112900.012.9613452
CGGGGAT62050.011.9458981
ATAATCC155850.011.6117688
GGGGAAT52900.010.5091161
TCTAGAC102650.09.6709147
CCGGGAT78350.09.5819551
CGAGGTC154750.09.5798771
GTTTAAT218900.09.5074571
TCATAAT191850.09.4593276
CCCTAAG108300.09.34181654-55
CGGGAAT95800.09.324561
GGGGATC49000.09.3082582
CTAGACT102800.09.2871648
CGGGGGT33050.09.2012011
CGGGGAG68900.09.0341661
GACCCTA55450.08.9943127
Produced by FastQC (version 0.11.2)