FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort4.351000000f3985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort4.351000000f3985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18038334
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA62750.016.1265034
TAATCCC81050.012.7171099
GTCATAA89450.011.4719735
AGGTCAT75400.011.02683
CGGGGGG36600.010.3858851
CCGGGAG97350.09.7617711
CGGGAAT84300.09.638361
CGGGCAT51150.09.568111
GGGGGAT41150.09.3529751
GAGGTCA112150.09.0657922
GGGGAAT47750.09.0552951
CGGGGTT32900.08.6654271
GGGGATC51550.08.6634412
ATAATCC122350.08.6573028
TCTAGAC79600.08.5927297
GGGGCAT28850.08.5643041
CCCGGAT50000.08.2676841
CGGGTAT55100.08.1923141
CTAGACT82200.08.1475878
GGGAATC64050.08.01116852
Produced by FastQC (version 0.11.2)