FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort3.351000000f396b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort3.351000000f396b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18257229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA70400.018.2882754
TAATCCC91850.013.548879
GTCATAA101850.012.9206085
AGGTCAT85700.012.1963163
GGGGAAT55500.011.1297651
CGGGGAT66500.010.7892411
GAGGTCA100100.010.4895222
GGGGGAT44100.010.0202831
GGGGATC52450.09.96425152
CGGGGAG69250.09.5374141
ATAATCC140050.09.2589238
CGGGCAT54550.08.9717681
CGGGAAT92700.08.7137431
CCCTAAG81000.08.70807954-55
CCGGGGT32200.08.7062621
CCCGGAT54750.08.6786361
TCTAGAC86100.08.49568757
GTTTAAT188800.08.4813211
CGGGTAT57800.08.3850941
CGGGGTT40800.08.3850941
Produced by FastQC (version 0.11.2)