FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort2.351000000f3935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort2.351000000f3935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15538822
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA62950.017.1318264
TAATCCC87600.012.254229
AGGTCAT71900.012.1583623
GTCATAA97700.011.3785375
GAGGTCA81650.010.5904042
GGGGAAT41950.010.1941541
CGGGAAT78400.010.0608031
CTAGACT82150.010.0605798
TCTAGAC84650.09.595127
CCGGAAT62250.09.38872051
GGGGTAT19750.09.3829221
CCGGGAT65200.09.2554341
ATAATCC123250.09.1721548
CGGGGCT26200.09.0679551
GGGGGAT34700.09.0376411
CGGGGAT63250.08.7895361
GGGCAAT48500.08.6214751
GTTTAAT175200.08.5973741
CGGGGAG56650.08.3876581
ATTCTAG112450.08.3650965
Produced by FastQC (version 0.11.2)