FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02n01_antort1.351000000f3928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02n01_antort1.351000000f3928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15689637
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA56300.018.4802954
ACTCTAG45800.014.3145775
AGGTCAT67150.012.7353063
TAATCCC77800.012.5767419
GAGGTCA71250.012.3362232
CCTTAGA58550.011.681979
GTCATAA87750.011.6400875
CTCTAGT50400.011.4056426
CTAGTTG46800.011.3671788
CACCTTA59950.011.2507037
CGTACAA169200.011.2311575
TTCGTAC159150.010.7766423
GGGGAAT45950.010.6512621
GTTTAAT143700.010.0192511
TCTAGTT63250.09.6123577
CGGGAAT73050.09.4969021
TCGTACA187500.09.4764034
TTTCGTA195900.09.2160432
CGGGTAT52650.09.205581
CTTTCGT210500.09.0067921
Produced by FastQC (version 0.11.2)