FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb8.351000000f3aae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb8.351000000f3aae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13449433
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA41800.012.5014784
GGGGAAT42800.011.9883341
CTGGGAT38750.010.2987951
CGGGGAT56700.010.1386741
CGGGAAT72300.09.3967271
AGGTCAT45050.09.3851263
GGGAATC54450.09.1608622
TAATCCC61050.09.0252819
GAGGTCA51100.09.01772
CTCGGAT74700.08.9676231
CGGGGTT30050.08.8536511
TCTAGAC53750.08.8371037
CGGGCCT29100.08.8161621
GTCATAA64850.08.790525
CTAGACT50550.08.7387688
CGGGCAT38150.08.7173071
GGGGGAT37350.08.3952221
CTGGAAT64350.08.342741
CGGGGCT31350.08.3349691
GGGGATC38900.08.0600792