FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb5.351000000f3a41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb5.351000000f3a41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600432
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA47250.014.27693754
GGGGAAT39950.011.1794051
CGGGGAT54850.010.7411961
CTGGGAT42200.010.6959341
AGGTCAT51600.010.6796453
CTGGGGT18650.010.1903411
TAATCCC63850.010.0429369
TCTAGAC69200.09.8842617
CTAGACT70800.09.7306611
GTCATAA70450.09.575345
GAGGTCA57550.09.4929472
CGGGCAT40700.09.339061
CCGGGAT51000.09.316171
CGGGAAT74950.09.2552641
CTCGGGT38450.09.0205721
CGGGGCT32000.08.9085871
CGGGGAG57750.08.8854481
CTGGGTT21950.08.8747671
GGGGCAT15900.08.6657951
CCGGGGT23950.08.5304221
Produced by FastQC (version 0.11.2)