FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb4.351000000f3a27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb4.351000000f3a27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13411955
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49250.016.7835854
AGGTCAT52850.013.8425923
GAGGTCA58150.013.3978732
TAATCCC68750.012.021679
GTCATAA75150.011.31520755
CGGGGAT53950.011.095621
CGGGAAT71350.010.9199921
TCTAGAC65300.010.2563837
CTGGGAT38800.010.1629281
CTGGAAT68350.09.9396251
CTAGACT64400.09.8834198
CCCGGAT34650.09.5977021
CGGGGCT31300.09.4106541
CGGGCAT36400.09.2667921
CCGGGAT44950.09.1952171
CGGGTAT38700.09.0843361
CATTCTA92600.08.9777764
CCCGGTT25950.08.7877231
GGGGGAT31900.08.6379321
CGAGGTC98200.08.5147841
Produced by FastQC (version 0.11.2)