FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb3.351000000f39ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb3.351000000f39ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13278784
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA41450.013.0650114
GGGGAAT40650.011.4538691
CCGGGAT47700.010.8566251
CGGGAAT68650.010.5885761
CGGGGAT51200.010.4856461
TCTAGAC58050.010.3917837
CTAGACT56500.010.2565188
CGGGTAT35700.010.2472941
CGGGCAT39900.010.0021431
CCCGGAT33350.09.8296931
CGGGGAG56400.09.4346461
TAATCCC60100.09.2469819
CCCGGGT20900.09.0928571
ATTCTAG75250.09.0273235
GAGGTCA52250.09.0007422
CGGGGTT25400.08.9783021
GGGGGAT32800.08.8357351
CGGGGCT31300.08.8038031
AGGTCAT50500.08.7482463
CATTCTA80950.08.685094