FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb2.351000000f39e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb2.351000000f39e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15522634
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC219540.14143218219279022TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA60050.017.8783634
AGGTCAT67500.013.7234463
GAGGTCA71350.012.9163582
TAATCCC83850.012.6325439
GTCATAA87100.012.32597355
GTATGCC101300.012.097644-45
CGGGGAT58000.011.959351
TATGCCG106750.011.94717946-47
TGCTTGA118700.011.04453258-59
CGGGAAT78250.010.9287591
GCCGTCT113500.010.8809448-49
ATGCCGT115450.010.63544346-47
CCGTCTT121700.010.577126550-51
CGTATGC122500.010.46927544-45
CCGGGAT55900.010.1988721
TAGCTTA123350.010.18533934-35
ACTAGCT125550.010.00686332-33
TCTAGAC81400.09.97839457
CGGGTAT45900.09.9366791
CTAGCTT124250.09.84395932-33
Produced by FastQC (version 0.11.2)