FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antormb1.351000000f39bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antormb1.351000000f39bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535828
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA52250.017.3654964
AGGTCAT59350.012.32650853
GAGGTCA61950.012.0392232
TAATCCC77300.011.7980479
CGGGGAT60050.011.2348831
GTCATAA84000.010.5189155
GGGGAAT42900.09.7458251
CCGGGAT57350.09.6927191
CCCGGAT41350.09.6515481
CGGGAAT79450.09.5081591
CGGGTAT41650.09.1257421
GGGGGAT40900.09.0607581
TATGCCG91150.08.93708446-47
GGGGGCT16700.08.5348911
GTATGCC86000.08.53332744-45
CTGGGTT24200.08.44201851
TGCTTGA100700.08.37254158-59
GTTTAAT150600.08.360151
CCCGGGT26750.08.347711
CTGGGAT42150.08.3411771
Produced by FastQC (version 0.11.2)