FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral8.351000000f3992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral8.351000000f3992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088445
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA48050.019.081164
AGGTCAT54850.013.5976513
TAATCCC63800.013.4011049
GTCATAA71200.012.8103835
GAGGTCA60050.012.26195052
CGGGGAT49300.012.0466661
CTAGACT59600.010.4430851
CGGGTAT35200.010.2582841
CGGGAAT63250.09.9906761
GGGGAAT35450.09.7838641
GGGGATC35300.09.689442
CGGGGAG51100.09.6697711
ATAATCC94650.09.5350238
CCGGGAT44550.09.4917441
CCCAATT68600.09.4885581
CGGGGTT24550.09.4830561
GGGGTAT19950.09.0499131
GGGGCAT17550.08.9338891
CGGGGGT30050.08.8541591
CTGGGAT38150.08.7178081
Produced by FastQC (version 0.11.2)