FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral7.351000000f3978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral7.351000000f3978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12826827
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT139560.10880321376440176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA45650.016.2340984
AGGTCAT52500.012.1252253
CGGGGAT45150.011.4706831
GGGGAAT47450.010.7144061
GAGGTCA59450.010.6278182
TAATCCC69350.010.6163139
GTCATAA71900.010.5713485
CCCGGAT41900.010.2058471
CCGGGAT46100.09.99749851
CGGGAAT64600.09.8558221
GGATAGT69800.09.734221
CGGGTAT35250.09.7049641
CGGGCAT35650.09.5960721
CGTACAA152750.09.3299495
TTCGTAC143750.09.2863033
GGGGATA29250.09.2574922
ATTATAG107250.08.9473285
TAGTTAA74900.08.8161164
GATAGTT73050.08.779262
TAGATAT117350.08.7440983
Produced by FastQC (version 0.11.2)