FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral6.351000000f395f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral6.351000000f395f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13159005
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGATCGAACGGGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGG211390.16064284495674255No Hit
GGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGGTCGGCGATGTCCC160480.1219545094784902No Hit
AAGAAACCCAAAAGATCGAACGGGGAGATTCATCGTCAGCGGCGCTGGCT158430.1203966409314382No Hit
CGGGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGGTCGGCGATGTC136750.10392123112651754No Hit
GCAAAACCCAAAGGCGCAATGAAAGTGAAGGTCGGCCTTGGCGCCGACCG133490.10144384016876656No Hit
CTGATACCGTGCGAGTACCGCCGGGCAGCCGGCCGAGCTTCCGGGGGTTT132070.10036473122397932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA32300.024.8554364
CCTCACC34000.024.5928541
CTAACAT57250.023.650731
CTCACCA36050.022.9287932
AGGCCCA40050.022.2969138
CATGTGC58950.021.9189135
TAACATG60750.021.3478222
CCCCAGT56850.020.4743821
TCACCAG43200.019.683693
ACATGTG68250.019.1411154
CACCAGG45300.019.08584
CCGTCAT44750.018.3649713
ATGTGCG78800.018.20586
CCCCCAT55650.018.0986391
GAGGTCA38700.017.5534422
GTCGGAT76050.017.3668381
CATTGAC49550.016.8716817
CCCAGTC71050.016.8489842
AACATGT79050.016.7062953
GGGTCCT33950.016.3713052
Produced by FastQC (version 0.11.2)