FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral5.351000000f3942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral5.351000000f3942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13661720
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA51150.016.3457684
TAATCCC69900.011.0084679
AGGTCAT59050.010.86055853
CGGGGAT51750.010.833421
GTCATAA78200.010.6915985
GAGGTCA66750.010.1770772
GGGGGAT31550.09.9389041
CTAGACT64350.09.8935051
GGGGAAT41300.09.7782881
CGGGGAG57700.09.3045751
CGGGTAT39550.09.0096661
GGGGATC39750.08.9631872
CGGGAAT72450.08.9185541
GGATAGT64100.08.894411
CGGGCAT44200.08.5992711
GACCCTA30450.08.4235567
CTGGAAT67300.08.40091
TCTAGAC66000.08.3483967
CCCAATT74650.08.3374921
GGGAATC54750.08.2428582
Produced by FastQC (version 0.11.2)