FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral4.351000000f391b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral4.351000000f391b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13873810
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT141390.10191144321567039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA62100.021.7259654
TAATCCC87950.015.7161199
AGGTCAT72100.015.5499533
GTCATAA91150.015.1663975
GAGGTCA75500.014.9755342
CGGGGAT51750.011.5679911
ATAATCC125950.011.4647248
GGATAGT75200.010.23516751
CGAGGTC115800.010.0520651
GGGGAAT42150.09.9193421
CGTACAA168150.09.8599495
GGGGGAT32600.09.7646111
GGGGTCA27850.09.7230392
TTCGTAC164200.09.6343723
CGGGGAG53100.09.5738591
GTTTAAT162500.09.5607541
TCATAAT147700.09.4880446
CGGGAAT69350.09.4543221
ATTATAG131700.09.4506485
CCGGGAT51100.09.3907051
Produced by FastQC (version 0.11.2)