FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral3.351000000f38f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral3.351000000f38f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12312791
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT129420.10511020612629582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49300.017.3446524
TAATCCC66200.013.3457759
GTCATAA69500.012.8502925
AGGTCAT57600.012.0412163
GAGGTCA61750.011.6935562
ACTCTAG26650.011.4083475
CGTACAA143900.010.464985
CGGGGAT48850.010.4062551
TTCGTAC137250.010.34900953
CGGGTCT41100.010.0566351
GTTTAAT120850.09.3956431
GGGGATC33550.09.3452612
TCGTACA156850.09.2678044
ATAATCC97500.09.2563098
TTTCGTA161450.09.0625952
CGGGCAT38350.09.0434211
TACAAAT189200.08.8371127
CTTTCGT181200.08.6785081
TAAGCCA43900.08.60208790-91
GGGGGAT29050.08.3406381
Produced by FastQC (version 0.11.2)