FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral2.351000000f38d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral2.351000000f38d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11627224
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49700.017.300834
AGGTCAT54400.013.18629553
TAATCCC67050.013.1765869
GAGGTCA57700.012.9261322
GTCATAA70500.012.4659555
CGGGGAT46150.09.9859991
CGGGTCT40100.09.8338861
CGGGAAT60500.09.6592011
ATAATCC95000.09.5998938
GGGGCTA10002.6557245E-109.5011192
CCCGGAT38300.09.42771151
CTAGACT63800.09.3829921
CGGGGAG47300.09.2409831
CGGGTAT33500.09.2184951
GGATAGT56400.09.0977951
GACCCTA32500.09.0614377
GGGGGAT27900.09.0253311
GTTTAAT128350.08.8099321
CCGGGAT46550.08.6754251
CCCGTAT28200.08.4238831
Produced by FastQC (version 0.11.2)