FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01n01_antoral1.351000000f38c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01n01_antoral1.351000000f38c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823180
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC321200.23236332016222028TruSeq Adapter, Index 1 (100% over 50bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT178880.12940582413019291No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA175480.12694618749086678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG86700.026.7937815
CTCTAGT93950.024.7256036
TCTAGTT107850.021.316357
TATGCCG110250.020.2922446-47
GGTCATA59600.020.2457224
CTAGTTG115000.019.5779958
GTACTCT119100.019.1857643
CGTATGC119450.018.888444-45
TACTCTA125350.018.6460384
CACCTTA99150.018.6380775
TGTACTC127650.017.9379182
CCGTCTT128150.017.5319550-51
CCAGTCA129150.017.41458926-27
GTATGCC103550.017.4080244-45
AGTCACA131400.017.2248428-29
GAGCACA213250.017.1287219
TGCTTGA130650.017.10558358-59
CCTTAGA108450.017.081377
ACTACCC51800.016.782486
TAGTTGT138000.016.5903539
Produced by FastQC (version 0.11.2)