FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7254027
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC85000.11717629393990399TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC80570.11106934120868313TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG685500.063.9897411
CACACGT686800.063.6746212
ACACGTC662750.063.57718713
ACGTCTG664200.063.3181615
CACGTCT660650.063.15146614
CGTCTGA665450.063.05961216
AGCACAC709300.062.64920410
GAACTCC680300.062.10238621
AGAGCAC732750.061.948848
ACTCCAG661250.061.73668323
CTGAACT674650.061.57663719
GAGCACA734950.061.4547239
GATCGGA748250.061.2578431
CGGAAGA761100.061.2394644
TCTGAAC682000.061.2178318
GTCTGAA698050.061.17733417
TCGGAAG754250.061.140083
GAAGAGC754400.061.1166156
TGAACTC697400.061.01949320
CTCCAGT652150.061.00050724
Produced by FastQC (version 0.11.5)